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rat jagged1 elisa kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rat jagged1 elisa kit
    ( A ) Endogenous mRNA expression of <t>Jag1</t> in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of <t>Jagged1</t> in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .
    Rat Jagged1 Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat jagged1 elisa kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    rat jagged1 elisa kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis"

    Article Title: RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis

    Journal: EMBO Reports

    doi: 10.1038/s44319-024-00356-7

    ( A ) Endogenous mRNA expression of Jag1 in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of Jagged1 in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .
    Figure Legend Snippet: ( A ) Endogenous mRNA expression of Jag1 in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of Jagged1 in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .

    Techniques Used: Expressing, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Control, Comparison, Staining, Recombinant

    ( A ) qPCR analysis of mRNA expression of Jag1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons. ** P = 0.0015, **** P < 0.0001. ( B ) ELISA measurement of secretory Jagged1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (* P = 0.0106, **** P < 0.0001). ( C ) IF staining of αSMA and BODIPY in HSCs treated with plain William’s E medium (control), conditioned medium (CM) collected from BEC Rage WT or KO cells, or CM collected from BEC Rage WT treated with siNeg, siJag1 #1 or siJag1 #2 for 72 h. Scale bar = 20 μm. .
    Figure Legend Snippet: ( A ) qPCR analysis of mRNA expression of Jag1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons. ** P = 0.0015, **** P < 0.0001. ( B ) ELISA measurement of secretory Jagged1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (* P = 0.0106, **** P < 0.0001). ( C ) IF staining of αSMA and BODIPY in HSCs treated with plain William’s E medium (control), conditioned medium (CM) collected from BEC Rage WT or KO cells, or CM collected from BEC Rage WT treated with siNeg, siJag1 #1 or siJag1 #2 for 72 h. Scale bar = 20 μm. .

    Techniques Used: Expressing, Negative Control, Comparison, Enzyme-linked Immunosorbent Assay, Staining, Control

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used: Recombinant, Staining, Plasmid Preparation, Antibody Labeling, Sequencing, Negative Control, RNAscope, Modification, Saline, Software, Transfection, Enzyme-linked Immunosorbent Assay, Microscopy, Mass Spectrometry, Real-time Polymerase Chain Reaction



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    rat jagged1 elisa kit  (Thermo Fisher)
    90
    Thermo Fisher rat jagged1 elisa kit
    ( A ) Endogenous mRNA expression of <t>Jag1</t> in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of <t>Jagged1</t> in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .
    Rat Jagged1 Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat jagged1 elisa kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    rat jagged1 elisa kit - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Endogenous mRNA expression of Jag1 in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of Jagged1 in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .

    Journal: EMBO Reports

    Article Title: RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis

    doi: 10.1038/s44319-024-00356-7

    Figure Lengend Snippet: ( A ) Endogenous mRNA expression of Jag1 in Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. Two-tailed t test was used for statistical comparisons (* P = 0.0222). ( B ) ELISA measurement of Jagged1 in BEC basal culture medium (Control) and conditioned medium (CM) collected from Rage WT and KO BECs. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (**** P < 0.0001). ( C ) Representative images of IF staining of αSMA and BODIPY in HSCs treated with PBS control or 1 μg/ml of recombinant Jagged1 (rJAG1). n = 3 independent experiments were performed. Scale bar = 20 μm. .

    Article Snippet: Rat Jagged1 ELISA Kit , Thermo Fisher Scientific , ERA35RB.

    Techniques: Expressing, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Control, Comparison, Staining, Recombinant

    ( A ) qPCR analysis of mRNA expression of Jag1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons. ** P = 0.0015, **** P < 0.0001. ( B ) ELISA measurement of secretory Jagged1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (* P = 0.0106, **** P < 0.0001). ( C ) IF staining of αSMA and BODIPY in HSCs treated with plain William’s E medium (control), conditioned medium (CM) collected from BEC Rage WT or KO cells, or CM collected from BEC Rage WT treated with siNeg, siJag1 #1 or siJag1 #2 for 72 h. Scale bar = 20 μm. .

    Journal: EMBO Reports

    Article Title: RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis

    doi: 10.1038/s44319-024-00356-7

    Figure Lengend Snippet: ( A ) qPCR analysis of mRNA expression of Jag1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons. ** P = 0.0015, **** P < 0.0001. ( B ) ELISA measurement of secretory Jagged1 in Rage WT or KO BECs, or Rage WT BECs treated with siRNA negative control (siNeg Ctrl), or siJag1 clone 1 (siJag1 #1) or siJag1 clone 2 (siJag1 #2) for 72 h. Data are shown as mean ± s.d. of n = 3 independent biological replicates. One-way ANOVA with Turkey’s multiple comparison test was used for statistical comparisons (* P = 0.0106, **** P < 0.0001). ( C ) IF staining of αSMA and BODIPY in HSCs treated with plain William’s E medium (control), conditioned medium (CM) collected from BEC Rage WT or KO cells, or CM collected from BEC Rage WT treated with siNeg, siJag1 #1 or siJag1 #2 for 72 h. Scale bar = 20 μm. .

    Article Snippet: Rat Jagged1 ELISA Kit , Thermo Fisher Scientific , ERA35RB.

    Techniques: Expressing, Negative Control, Comparison, Enzyme-linked Immunosorbent Assay, Staining, Control

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis

    doi: 10.1038/s44319-024-00356-7

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Rat Jagged1 ELISA Kit , Thermo Fisher Scientific , ERA35RB.

    Techniques: Recombinant, Staining, Plasmid Preparation, Antibody Labeling, Sequencing, Negative Control, RNAscope, Modification, Saline, Software, Transfection, Enzyme-linked Immunosorbent Assay, Microscopy, Mass Spectrometry, Real-time Polymerase Chain Reaction